LABORATORY TESTS

UNDER CONSTRUCTION - MORE COMING NEXT!

Based on my own Lyme and Chlamydia infection, my survey and statistical data collected from Morgellons afflicted, I must claim that 2/3 of the Morgellons afflicted are actually suffering from Lyme disease and its coinfections, and the remaining 1/3 had no or some typical Lyme symptoms, but disappointly they had a seronegative result.

But this fact is well known that Lyme disease very often does not show usual clinical findings. This can happen with Borrelia and many other pathogens which are not always present in the bloodstream, because they are already located intracellularly or incapsulated as cystic forms somewhere in the connective tissue or have changed their surface proteins.

If you have heavy cough, pneumonia and cystic fibrosis-like symptoms and a lot of mucous is coming up, then this can be caused not only by Borrelia bacteria but also by enterobacteria as E. coli, Klebsiella oxytoca which could be carried from borrelia bacteria leaving the guts and entering the blood system and so on. At least you should test all the "pneumoniae" variants of pathogens which may cause lung issues.

If you want to prove any fungal infection, if culturing did fail, also an automated fluorescent amplified fragment length polymorphism (AFLP) method can be used to examine fungal DNA. An immunofluorescent test can also detect a fungus if usual cultures did fail. Another bacterial & fungal Identification could be done by "Comparative 16s rDNA Sequencing"

The main pathogens appearing with Morgellons or Lyme disease are listed below.

What pathogens should be tested!

Laboratory diagnostics of the Borrelia infection, requested by a physician experienced with Lyme disease, are always indicated when a patient's complaints or clinical findings are compatible with a Lyme infection. Serological testing results, for the purpose of evaluating the success of therapy, are not meaningful for chronic Lyme, therapy success must be judged clinically.

Under strictly scientific criteria, the cultural proof of Borrelia infection can only be demonstrated through pathogen DNA-identification by polymerase chain reaction (PCR) methods. The proof of Borrelia DNA by means of PCR nuclear confirmation is likewise of high importance.

Borrelian serology is the basis diagnostic for the question of whether a Borrelia infection could be present; however the testing methods presently on the market, the enzyme-linked immunosorbent assay (ELISA) and immunoblot (Western blot) a test for immunoglobulins, or antibody proteins, signified by the initials IgG and IgM, are not standardized. Therefore, findings from these two methods can be compared only at the lowest common standards among them, and crossreactions caused from other pathogens with similar cell surface proteins can happen!

The investigation of the presence of Borrelia-specific antibodies is possible only by means of immunoblot. With suspicion of one Borrelia infection, the IgG and IgM immunoblot Borrelia test should be required in all cases. The laboratory to which samples are referred must be required to express results in Borrelia serology inclusive of immunoblot for Borrelia. Even so, these tests can show negative results that do not exclude a true infection. One reason for a negative result can be that the antigen spectrum in an immunoblot assay did not match identically the antigens found in ELISA results.

Borrelia ELISA and immunoblot are two different test methods, which correlate to a considerable degree with one another, but with different results in some cases. Negative serology does not exclude a present Borrelia infection, and even in the absence of antibodies, the need for treatment must be considered urgent. Seronegativity can be caused by early but inadequate antibiotic treatment, therapies with immunosuppressants, higher cortisol levels, exhaustion of the immune system, hidden pathogens, and a genetic disposition.

On the other hand, positive serological findings just mean that the patient acquired a Borrelia infection somewhere, at some point, with older infections evidenced by IgG and more recent ones by IgM. It is not possible to decide whether this infection is latent or active by a unique serological investigation. That can be decided only by the treating physician on basis of an individual patient's clinical process and symptoms.

The table below shows different Borrelia protein antigens/antibodies which can be detected by serology.

Protein antigen

p14, 18

p19

p21

Decorin binding protein particulary on skin and tissue cells of the host

p22, 23, 24, 25

Most important IgM response marker of an early infection stage. Known are 13 different OspC-types

p26

p29

p31

Known are 7 different OspA-types which defines different species

p34

Antibody appears more in a later infection stage

p39

Antibody appears more in an early infection stage

p41

Primary and early appearing IgM antibodies. Cross-reactions with other kinds of spirochete and bacteria with flagella are possible

p58

p60

Antibodies which often might be present also with other bacterial infections

p66

Antibody reaction to any bacterial infection

p75

p83, 100

Antibodies mainly present in a later infection stage

VlsE

VlsE protein is expressed only in the host. IgG-Antibodies might be present already in an early infection stage.

What can you do if tests were seronegative?

These pathogens can be encapsulated into human tissue by created cysts, to avoid detection and measurements and to survive there inside the cysts for a prolonged time, and also therefore no clinical results or antigens can be found and also no antibodies have been created from the immune system to detect and fight pathogens. Same situation can happen with the cell wall deficiency Borrelia forms!

Don`t dispair, this can happen very often and an experienced physician based upon the symptoms will prescribe you then the right medication. If you don`t have any insurance or medical assistance, many sufferers reported us that they have ordered medications over online pharmacies. Please take care, many companies sell just fake drugs and without any physician you might not come further to treat such heavy infections!

Check below different tests to detect Lyme disease.

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